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Ball Milling Formula

Lso2 is a conserved ribosome.Frozen cells were lysed by ball milling in a retsch cryomill one minute of milling at 10 hz followed by 1 minute of cooling was repeated 10 times the lysate was spun for 5 minutes at 3 000 g to remove debris the supernatant was removed and further clarified for 10 minutes at 21 000 g the od 260 of each sample was determined by nanodrop.

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